VTCDDSL Instrumentation

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VTCDDSL Instrumentation 2017-03-13T16:10:42+00:00

Instrumentation for High-Throughput Analysis and Drug Screening

All facility instruments are compatible with 96-well and 384-well microplates. It may be possible to use other plate types for select instruments.

Biotek Multiflo Microplate Dispenser

The Biotek Multiflo is configured with two peristaltic cassette dispensers, and two syringe dispensers, allowing at least 4 reagents to be dispensed simultaneously. A single reagent can be dispensed with each syring pump and up to 8 reagents (one per plate row) can be dispensed using the peristaltic pump cassettes. The Multiflo is equipped with a Biostak multiplate stacker.
2 syringe pumps                   1-3000 ul
2 peristaltic cassettes          1-3000 ul
Plate type                             96 and 384 well
Capacity                               30 plates

Agilent Bravo Automated Liquid-Handling Platform

The Agilent Bravo is a versatile, high-precision liquid-handling system that enables plate-to-plate liquid transfers, among other tasks. It is equipped with 96ST head, allowing for the use of disposable pipette tips, and a tip-washing station, allowing the cost-saving reuse of pipette tips. It can process batches of up to 4 plates, although batches of 8 or 12 plates may be possible with certain procedures.
Volume range                  0.3–70 ul
Plate type                        96 and 384 well
Capacity                          4 plates

Molecular Devices SpectraMax M5 Multi-Mode Plate Reader

The SpectraMax M5 is a multi-mode plate reader equipped with the StakMax plate stacker that can accomodate 50 plates. It allows for both top-and bottom- read modes.
Modes                             UV-Vis Absorbance
                                          Fluorescence Intensity
                                          Time-Resolved Fluorescence
                                          Fluroescence Polarization
                                          Glow Luminescence
Plate Type                       6, 12, 24, 48, 96, and 386 well
Plate Capacity                50 plates
Shaker                             Yes
Temperature                  Ambient to 37 degrees C

Waters Acquity UPLC

The facility has the following UPLC columns available for use:
ACCQ-TAGTM ULTRA C18, 1.7 um, 2.1×100 mm
BEH C18, 1.7 um, 2.1 x 50 mm
BEH C18, 1.7 um, 2.1 x 100 mm
Maximum Pressure
        flow rates <1 ml/min                           18,000 psi
        flow rates <2 ml/min                           12,000 psi
Sample Volume                                            0.1-10 ul
Sample Capacity                                          2 microplates
Sample compartment temperature             4-40 degrees C
Column temperature                                   20-90 degrees C
Detector                                                      Tunable UV

Heidolph Microplate Shaker

This custom-made, 17-plate shaker is useful for many protocols

BSL2 Equipment

For BSL2 work, the lab has a 5′ biosafety cabinet, a CO2 Incubator, and sealed microplate rotors.

Contact the Screening Lab

Dr. Pablo Sobrado
VTCDDSL Director, Associate Professor
Department of Biochemistry
(540) 231-9485
psobrado@vt.edu

Dr. Nancy Vogelaar
VTCDDSL Manager
Department of Biochemistry
(540) 231-3525
nancy.vogelaar@vt.edu

Location
The Virginia Tech Center for Drug Discovery Screening Lab is located in Engel Hall, Room 303. The facility is available to the Virginia Tech community for high-throughput screening and users have access to over 40,000 chemical compounds.