Screening Lab

photo of Drs Sobrado, Vogelaar, and Kingston in screening lab
Drs. P. Sobrado, N. Vogelaar, and D.G.I. Kingston

Contact the Screening Lab

Dr. Pablo Sobrado
VTCDDSL Director, Professor
Department of Biochemistry
(540) 231-9485
psobrado@vt.edu

Dr. Nancy Vogelaar
VTCDDSL Manager
Department of Biochemistry
(540) 231-3525
nancy.vogelaar@vt.edu

Location
The Virginia Tech Center for Drug Discovery Screening Lab is located in Engel Hall, Room 301. The facility is available to the Virginia Tech community for high-throughput screening and users have access to over 40,000 chemical compounds.

Instrumentation for High-Throughput Analysis and Drug Screening

All facility instruments are compatible with 96-well and 384-well microplates. It may be possible to use other plate types for select instruments.

Biotek Multiflo Microplate Dispenser

The Biotek Multiflo is configured with two peristaltic cassette dispensers, and two syringe dispensers, allowing at least 4 reagents to be dispensed simultaneously. A single reagent can be dispensed with each syring pump and up to 8 reagents (one per plate row) can be dispensed using the peristaltic pump cassettes. The Multiflo is equipped with a Biostak multiplate stacker.

  • 2 syringe pumps                   1-3000 ul
  • 2 peristaltic cassettes          1-3000 ul
  • Plate type                             96 and 384 well
  • Capacity                               30 plates

Link to Manufacturer
Demonstration Video

Agilent Bravo Automated Liquid-Handling Platform

The Agilent Bravo is a versatile, high-precision liquid-handling system that enables plate-to-plate liquid transfers, among other tasks. It is equipped with 96ST head, allowing for the use of disposable pipette tips, and a tip-washing station, allowing the cost-saving reuse of pipette tips.

  • Volume range                  0.3–70 ul
  • Plate type                        96 and 384 well

Link to Manufacturer
Link to Demonstration Video

Molecular Devices SpectraMax M5 Multi-Mode Plate Reader

The SpectraMax M5 is a multi-mode plate reader equipped with the StakMax plate stacker that can accommodate 50 plates. It allows for both top- and bottom- read modes.

  • Modes                             UV-Vis Absorbance
  •                                           Fluorescence Intensity
  •                                           Time-Resolved Fluorescence
  •                                           Fluroescence Polarization
  •                                           Glow Luminescence
  • Plate Type                       6, 12, 24, 48, 96, and 386 well
  • Plate Capacity                50 plates
  • Shaker                             Yes
  • Temperature                  Ambient to 37 degrees C

Link to Manufacturer
Demonstration Video

Waters Acquity UPLC

The facility has the following UPLC columns available for use:

  • ACCQ-TAGTM ULTRA C18, 1.7 um, 2.1×100 mm
  • BEH C18, 1.7 um, 2.1 x 50 mm
  • BEH C18, 1.7 um, 2.1 x 100 mm

Maximum Pressure

  •         flow rates <1 ml/min                           18,000 psi
  •         flow rates <2 ml/min                           12,000 psi

Sample Volume                                            0.1-10 ul

Sample Capacity                                         768, 2 microplates

Sample compartment temperature             4-40 degrees C

Column temperature                                   20-90 degrees C

Detector                                                      Tunable UV

Link to Manufacturer

Heidolph Microplate Shaker

This custom-made, 17-plate shaker is useful for many protocols

BSL2 Equipment

For BSL2 work, the lab has a 5′ biosafety cabinet, a CO2 Incubator, and sealed microplate rotors.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The Spectrum Collection from MicroSource

The 2320 compounds in the SPECTRUM Collection were selected by medicinal chemists and biologists so as to provide a wide range of biological activities and structural diversity for screening programs with special emphasis on the de-novo assays emerging today.  Each compound has > 95% purity.

Two copies of the library are available on campus: one owned by VTCDD and one owned by Dr. Pablo Sobrado.

Drug components:          60%

Natural Products:            25%

Bioactive Components:   15%

Website: http://www.msdiscovery.com/spectrum.html

Number of Compounds   

 2320
Compound State  10mM in DMSO, 1mM in DMSO

 

Analyticon Discovery Screening Library (AD122131)

This library is an Academia subset of the Analyticon Discovery NATx Screening Library. The Academia library uses 70 chemical entities with biological pre-validation as underlying templates. These molecules are then modified with known and unusual pharmacophore groups using straightforward floow-up chemistry to produce 5,000 unique molecules for drug discovery. Compound purity averages 95 percent.

Product Information

 

Number of Compounds     5000
Compound State  0.5mg, lyophilized

 

Chembridge Library (#1032)

This library is a custom selection from the Chembridge DriverSet and belongs to Dr. Florian Schubot. He has generously allowed the VTCDDSL to prepare dilutions and these are available for general use.

Website:

http://www.chembridge.com/screening_libraries/diversity_libraries/

 

Number of Compounds     30,000
Compound State  10 mM in DMSO, 1mM in DMSO

 

 

The VTCDD Merola Tailored Transition Metal Complex (TTMC) Library

This library of unique organometallic compounds was prepared here at Virginia Tech by the Merola laboratory (Department of Chemistry, College of Science).  Compounds with these molecular frameworks can be easily modified and their properties “tailored” to improve performance.   A number of the library compounds have have been tested for toxicity and shown to be non-toxic to Vero cells.  The only library compound tested to date in a mouse study was also shown to be non-toxic.
Many compounds in the TTMC library have demonstrated anti-mycobacterial activity (1) and others have shown activity against Staphlococcus aureus including several strains of MRSA (2).

  • .Karpin, G.W., Merola, J.S., and Falkinham, J.O., III.  “Transition metal-α-amino acid complexes with antibiotic activity against Mycobacterium spp.” Antimicrobial agents and chemotherapy 2013, 57(7), 3434-3435.
    Link to article:http://aac.asm.org/content/57/7/3434.full.pdf+html
  • Karpin, G.W., Morris, D.M, Njo, M.T., Merola, J.S. and Falkinham, J.O., III.   “Transition metal diamine complexes with antimicrobial activity against Staphylococcus aureus and methicillin-resistant S. aureus (MRSA)”  Med. Chem. Comm. Med. Chem. Commun., 2015,6, 1471-1478.Link to article:
    http://pubs.rsc.org/en/content/articlepdf/2015/md/c5md00228a

     

    Number of Compounds  110
    Compound State  10 mM in DMSO, 1 mM in DMSO

 

 

Selleckchem Kinase Inhibitor Library (L1200)

This library is a unique collection of 273 structurally diverse inhibitors for kinases including RTKs, PI3K, Aurora Kinase, CDK, and MEK.  Most of these inhibitors are competitive with ATP.  The bioactivity and safety of these compounds have been confirmed by preclinical research and clinical trials.  Some of these inhibitors have been approved by the FDA.

Number of Compounds      273
Compound State  10 mM in DMSO

 

Brominated Fragment Library (Otava)

Fragment-based drug discovery is a method where very small molecules (“fragments”), about half the size of a typical drug, are screened for their ability to bind the target of interest.  The binding properties of identified fragments can then be improved through modification, preferably utilizing structural information.  The screening lab has purchased a 490-compound library of brominated compounds from OTAVA that can be used for fragment-based drug discovery.  The bromine atoms have been incorporated to facilitate the identification of binding sites in crystallographic screening methods, although they may also allow for more diverse chemical interactions with the target protein.

Number of Compounds      490
Compound State  > 100 mM in DMSO

 

Targetmol Natural Products Library

The Targetmol Natural Products Library contains compounds purified from a wide diversity of natural sources including microorganisms, plants, and animals.  The compounds are diverse, including saccharides, glycosides, alkaloids, phenols, terpenoids, flavonoids, and steroids.

Number of Compounds     1500
Compound State  10 mM in DMSO

 

Contact the Screening Lab

Dr. Pablo Sobrado
VTCDDSL Director, Professor
Department of Biochemistry
(540) 231-9485
psobrado@vt.edu

Dr. Nancy Vogelaar
VTCDDSL Manager
Department of Biochemistry
(540) 231-3525
nancy.vogelaar@vt.edu

Location
The Virginia Tech Center for Drug Discovery Screening Lab is located in Engel Hall, Room 301. The facility is available to the Virginia Tech community for high-throughput screening and users have access to over 40,000 chemical compounds.